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1.52026

Chromolith® NH2 (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® Flash NH2 25-4.6 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
NH2 bonding phase
Pore size:
13 nm mesoporosity, 2 μm macroporosity
Matrix:
monolithic silica
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Product Name

Chromolith® NH2, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

100

agency

suitable for USP L8

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

extent of labeling

5% Carbon loading

parameter

200 bar pressure, 45 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

2.5 cm × 4.6 mm

matrix

monolithic silica

matrix active group

NH2 bonding phase

pore size

13 nm mesoporosity, 2 μm macroporosity

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

storage temp.

no temp limit

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 25 mm length and 4.6 mm I.D. is the best choice for rapid separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Pressure: ≤ 5 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.35 %
Theoretical Plates (N/m) (Cytosine): ≥ 75000
Capacity factor (Cytosine): ≥ 1.0

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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