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Merck

HCYP2MAG-62K

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Synonym(s):

Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Product Name

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay, Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 81-110%
sensitivity: 0.4-55.8 pg/mL
standard curve range: 1.0-100,000 pg/mL

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Quality Level

200

Related Categories

Application

  • Analytes: 6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1δ, SCF, SDF-1A+β, TARC, TPO, TRAIL, TSLP
  • Recommended Sample type: Serum, plasma, cell culture supernatant
  • Recommended Sample dilution: neat
  • Assay Run Time: Overnight or two-hour primary incubation. For best results, an overnight incubation is recommended
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Obesity, Metabolic Disorders, Inflammation & Autoimmune Mechanisms

Biochem/physiol Actions

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

The MILLIPLEX® Human Cytokine / Chemokine Panel II enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Other Notes

Please contact Technical Service for linearity of dilution.

Packaging

96 well plate

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evidence of enhanced systemic inflammation in stable kidney transplant recipients with low Framingham risk scores.
Mansell, H; Rosaasen, N; Dean, J; Shoker, A
Clinical Transplantation null
Cecilia K Andersson et al.
The Journal of allergy and clinical immunology, 139(6), 1819-1829 (2016-10-18)
Neutrophils and IL-17A have been linked mechanistically in models of allergic airways disease and have been associated with asthma severity. However, their role in pediatric asthma is unknown. We sought to investigate the role of neutrophils and the IL-17A pathway
Fawaz Y Azizieh et al.
Journal of inflammation research, 12, 99-108 (2019-05-24)
Objective: In addition to some well-characterized bone turnover markers (BTMs), cytokines and adipokines have also been suggested to be linked to osteoporosis seen in menopause. However, there is much controversy on the possible association between these markers and bone mineral density
Yanping Tong et al.
Frontiers in neurology, 9, 44-44 (2018-03-03)
A prominent pathological feature of neuromyelitis optica spectrum disorders (NMOSD) is markedly greater eosinophilic infiltration than that seen in other demyelinating diseases, like multiple sclerosis (MS). Eosinophils express the chemokine receptor CCR3, which is activated by eotaxins (CCL11/eotaxin-1, CCL24/eotaxin-2, CCL26/eotaxin-3)
Ashok Kumar Kumawat et al.
Mediators of inflammation, 2014, 879843-879843 (2014-10-22)
Soluble factors from intestinal mucosal cells contribute to immune homeostasis in the gut. We have established an in vitro model to investigate the regulatory role of soluble factors from inflamed intestinal mucosa of collagenous colitis (CC) patients in the differentiation
View All Related Papers

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The Power of Biomarker Analysis
MAGPIX®and Luminex 200™ - Multiplex Detection of Inflammation Pathway Analytes

"Multiplex detection is a highly efficient tool for measuring the levels of multiple analytes in a single sample. MILLIPLEX® MAP assay panels provide a complete solution for multiplex detection of analytes within particular research areas using the bead-based Luminex® xMAP® technology. Previously, the only option for analyzing MILLIPLEX® MAP assays required the Luminex 200™ or FLEXMAP 3D® analyzers. With the launch of the MAGPIX® system, MILLIPLEX® MAP magnetic bead assays can be analyzed without the use of lasers or hydrodynamic focusing. The MAGPIX® instrument is a compact, cost-effective multiplexing system based on CCD imaging technology. The MAGPIX® instrument was specifically designed to yield comparable results to the Luminex 200™ instrument. To demonstrate similarity between the two systems, we ran parallel assays using the MILLIPLEX® MAP Human Cytokine/Chemokine Magnetic Bead Panel. Standard curve median fluorescence intensity (MFI) and sample range data are similar between the two instrument systems. Furthermore, sample concentration data between the MAGPIX® and Luminex 200™ systems demonstrate correlation coefficients ≥ 0.97."

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Global Trade Item Number

SKUGTIN
HCYP2SF-62K-19C04065267246437
HCYP2SF-62K-0904065267244105
HCYP2SF-62K-1904065267246208
HCYP2SF-62K-18C04065267246420
HCYP2SF-62K-06C04065267246307
HCYP2SF-62K-16C04065267246406
HCYP2SF-62K-22C04065267246468
HCYP2SF-62K-10C04065267246345
HCYP2SF-62K-01C04065267246253
HCYP2SF-62K-1604065267246178
HCYP2SF-62K-2004065267246215
HCYP2SF-62K-0404065267244051
HCYP2SF-62K-2104065267246222
HCYP2SF-62K-1404065267246154
HCYP2SF-62K-1704065267246185
HCYP2SF-62K-0204065267244037
HCYP2SF-62K-2304065267246246
HCYP2SF-62K-1504065267246161
HCYP2SF-62K-0804065267244099
HCYP2SF-62K-1804065267246192
HCYP2SF-62K-09C04065267246338
HCYP2SF-62K-08C04065267246321
HCYP2SF-62K-04C04065267246284
HCYP2SF-62K-14C04065267246383
HCYP2SF-62K-02C04065267246260

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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