YUMM3.2 Mouse Melanoma Cell Line

Immunocompetent genetically-engineered mouse models with select genetic mutations that drive melanoma are essential for studying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate models with appropriate genetic backgrounds.​SourceYUMM3.2 mouse melanoma cell line was derived from the Braf/Pten genetically engineered mouse melanoma model, a highly penetrant GEMM in which melanomas develop with short latency.​YUMM3.2 cells are syngenic with the immunocompetent C57Bl/6 mouse background and retain genetic markers of the Braf/Pten mouse model, characterized by activation of Braf and inactivation Cdkn2a. The YUMM3.2 cell line retains wild-type Pten, allowing for comparative analysis for the parsing of distinct contributions of PI3K-Akt signaling in melanoma tumorigenesis and immune evasion.References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that reiterate genetic drivers found in a significant proportion of human melanomas. YUMM3.2 cells are syngeneic with the immunocompetent C57Bl/6 mouse strain.

The YUMM cell lines recapitulate genetic drivers common in human melanomas. YUMM3.2 retains WT Pten, permitting comparative analysis for parsing distinct contributions of PI3K-Akt signaling in melanoma tumorigenesis and immune evasion.

YUMM3.2 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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