U1250
Urea
≥99.5%, pellets, ReagentPlus®
Synonym(s):
Carbamide, Carbonyldiamide
Sign In to View Organizational & Contract Pricing.
Select a Size
All Photos(2)
About This Item
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
200-315-5
MDL number:
Beilstein/REAXYS Number:
635724
Product Name
Urea, ReagentPlus®, ≥99.5%, pellets
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
SMILES string
NC(N)=O
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
product line
ReagentPlus®
assay
≥99.5%
form
pellets
mp
132-135 °C (lit.)
solubility
H2O: 8 M
density
1.335 g/mL at 25 °C (lit.)
functional group
amine
Quality Level
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
Looking for similar products? Visit Product Comparison Guide
Related Categories
Application
Urea has been used as a protein denaturing agent.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
General description
Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. Urea can also disturb hydrogen bonds present in DNA secondary structure.
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Christos D Georgiou et al.
Redox biology, 17, 128-142 (2018-04-24)
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
Sreenivasa C Ramaiahgari et al.
Archives of toxicology, 88(5), 1083-1095 (2014-03-07)
Immortalized hepatocyte cell lines show only a weak resemblance to primary hepatocytes in terms of gene expression and function, limiting their value in predicting drug-induced liver injury (DILI). Furthermore, primary hepatocytes cultured on two-dimensional tissue culture plastic surfaces rapidly dedifferentiate
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service