L2769
Lectin from Phaseolus vulgaris (red kidney bean)
Leucoagglutinin PHA-L, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris
biological source
Phaseolus vulgaris
form
lyophilized powder
potency
erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity
composition
Protein, ≥60% biuret
impurities
salt, free
storage temp.
2-8°C
Quality Level
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General description
Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.
Application
Lectin from Phaseolus vulgaris (red kidney bean) has been used:
- as a positive control in ELISPOT (enzyme-linked immunospot) assay
- to induce Th2 polarization for T-cell differentiation in vitro
- as controls in the lymphocyte proliferation assays
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Preparation Note
Purified by a modification of the method of Leavitt.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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