M9004
Malic Dehydrogenase from bovine heart
ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
Synonym(s):
L-Malate:NAD+ oxidoreductase, MDH
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart
biological source
bovine heart
Quality Level
form
ammonium sulfate suspension
specific activity
2000-4000 units/mg protein (modified Warburg-Christian)
mol wt
35 kDa
impurities
≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase
storage temp.
2-8°C
General description
Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Application
Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.
Physical form
Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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