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14-902 Sigma-AldrichAMPK (α2, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

AMPK (α2, β1, γ1) Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA

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Overview

Replacement Information

Description
Catalogue Number	14-902
Brand Family	Upstate
Trade Name	Upstate
Description	AMPK (α2, β1, γ1) Protein, active, 10 µg
Overview	Recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 and full length untagged AMPKγ1.

References

Product Information
Quality Level	MQ100

Applications
Application	Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.
Key Applications	Kinase Assay

Biological Information
Source	Expressed in E. coli cells and activated in vivo.
Specific Activity	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number	NM_006252 NM_006253 NM_002733
Entrez Gene Summary	The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Gene Symbol	PRKAB1 PRKAG1 PRKAA2
Protein Target	AMPK (α2, β1, γ1)
Purification Method	Cobalt-agarose
Target Sub-Family	CAMK
UniProt Number	P54646, Q9Y478, P54619
UniProt Summary	Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulation: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. Subunit structure: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Molecular Weight	AMPKα2 MW = 63 kDa, AMPKß1 MW = 30 kDa, AMPKγ1 MW = 38 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation of substrate peptide
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	6 months at -70°C

Packaging Information
Material Size	10 µg
Material Package	Also available in 250µg size as catalog # 14-902M - Call for pricing and availability.

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
14-902	04053252368035

Documentation

AMPK (α2, β1, γ1) Protein, active, 10 µg SDS

Title
Safety Data Sheet (SDS)

AMPK (α2, β1, γ1) Protein, active, 10 µg Certificates of Analysis

Title	Lot Number
AMPK (#945;2, #946;1, #947;1), active - 1637297	1637297
AMPK (#945;2, #946;1, #947;1), active - D8PN063N-A	D8PN063N-A

Related Products & Applications

Product Families

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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