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14-902M AMPK (α2, β1, γ1) Protein, active, 250 µg

Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

AMPK (α2, β1, γ1) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-902M
250 µg  Also available in 10 μg size (cat#14-902) and in bulk (cat# 14-902-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-902M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAMPK (α2, β1, γ1) Protein, active, 250 µg
OverviewRecombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 and full length untagged AMPKγ1.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli cells and activated in vivo.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Gene Symbol
  • PRKAB1
  • PRKAG1
  • PRKAA2
Protein TargetAMPK (α2, β1, γ1)
Purification MethodCobalt-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFunction: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
Subunit structure: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Molecular WeightAMPKα2 MW = 63 kDa, AMPKß1 MW = 30 kDa, AMPKγ1 MW = 38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-902) and in bulk (cat# 14-902-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-902M 04053252335211

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Alternative Packsize

Catalogue Number Description
14-902 AMPK (α2, β1, γ1) Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases