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05-389-AF488 Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate

This Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000
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05-389-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-389-AF488
DescriptionAnti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate
Alternate Names
  • Ras-related C3 botulinum toxin substrate 1
  • Cell migration-inducing gene 5 protein
  • Ras-like protein TC25
  • p21-Rac1
  • Rac1
Background InformationRas-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions, including cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family consists of 22 members, which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamily (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Biological Information
ImmunogenRecombinant protein containing the full length human Rac.
Clone23A8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
IsotypeIgG2b
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAC1
  • TC25
  • MIG5
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~21 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-389-AF488 04055977300819

Documentation

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-Rac1, clone 23A8, -Q2612308 Q2612308
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate 3043041
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate 2857472
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate - 3673693 3673693
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate - 4107257 4107257
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate - 4168125 4168125
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate - 4242014 4242014
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate -2859440 2859440
Anti-Rac1, clone 23A8, Alexa Fluor® 488 Conjugate -2860580 2860580

Brochure

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New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications