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16-319 Sigma-AldrichAnti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate

Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate detects level of Rac1 & has been published & validated for use in WB & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA

Research Category: Signaling Research Sub-Category: G-proteins, Cytoskeletal Signaling Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	16-319
Description	Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate
Alternate Names	Cell migration-inducing gene 5 protein Ras-like protein TC25 migration-inducing gene 5 migration-inducing protein 5 ras-related C3 botulinum toxin substrate 1 ras-related C3 botulinum toxin substrate 1 (rho family,small GTP binding protein Rac1) ras-related C3 botulinum toxin substrate 1 isoform Rac1 rho family, small GTP binding protein Rac1
Background Information	Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.

References

Product Information
Format	Purified
Presentation	20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 immunoprecipitation assays.
Quality Level	MQ100

Applications
Application	Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate detects level of Rac1 & has been published & validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation

Biological Information
Immunogen	Recombinant protein containing the full length human Rac1.
Clone	clone 23A8
Host	Mouse
Specificity	Recognizes human Rac1.
Isotype	IgG2b
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, Mouse, Rat. Other species have not been tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_008839
Entrez Gene Summary	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol	RAC1 TC25 MIG5
UniProt Number	P63000
UniProt Summary	Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Subunit structure: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH By similarity. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sideBy similarity. Melanosome. Note= Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine By similarity. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Domain: The effector region mediates interaction with DEF6. Sequence similarities: Belongs to the small GTPase superfamily. Rho family.
Molecular Weight	21 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. DO NOT FREEZE! Wash the magnetic beads with an appropriate buffer prior to use to remove sodium azide.

Packaging Information
Material Size	400 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
16-319	04053252292361

Documentation

Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate SDS

Title
Safety Data Sheet (SDS)

Anti-Rac1 Antibody, clone 23A8, Magnetic bead Conjugate Certificates of Analysis

Title	Lot Number
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 2251772	2251772
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 2544336	2544336
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 3147643	3147643
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 3639164	3639164
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 4017796	4017796
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 4217506	4217506
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate - 4292209	4292209
Anti-Rac1, clone 23A8, Magnetic Bead Conjugate Monoclonal Antibody	2918036
Anti-Rac1, clone 23A8,Magnetic Bead Conjugate - NRG1870325	NRG1870325

Related Products & Applications

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Catalogue Number	Description
05-389	Anti-Rac1 Antibody, clone 23A8
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05-389-AF647	Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate
07-1464	Anti-Rac1 Antibody
09-271	Anti-Rac1b Antibody

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies

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