Skip to Content
Merck

Key Documents

View All Documentation

1.52014

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 25 mm x 2 mm HPLC Column

Synonym(s):

Chromolith® Flash RP-18 endcapped 25-2 HPLC column

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Matrix active group:
C18 bonding phase
Pore size:
1.5 μm macroporosity, 13 nm mesoporosity
Matrix:
monolithic silica
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Chromolith® RP-18 endcapped, L × I.D. 25 mm x 2 mm HPLC column

material

PEEK column

Quality Level

100

agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

1 ea of

extent of labeling

18% Carbon loading

parameter

200 bar pressure, 45 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

2.5 cm × 2 mm

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

1.5 μm macroporosity, 13 nm mesoporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

General description

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Flash RP-18 endcapped 25-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Flash RP-18 endcapped 25-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® Flash RP-18 endcapped 25-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 2 mm I.D. is the best choice for rapid separations on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) " /product/mm/152009">1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Pressure: ≤ 20 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 70000
Capacity factor (Anthracene): 3.3 - 4.3
Tailing (USP): 0.90 - 1.40 %
separation factor (anthracene/progesterone): 2.2 - 2.6

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under Chromolith® RP-18 endcapped

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library