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B0126

Bromophenol Blue

Powder

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form

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About This Item

Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
115-39-9
Molecular Weight:
669.96
NACRES:
NA.47
PubChem Substance ID:
24891504
UNSPSC Code:
12171500
EC Number:
204-086-2
MDL number:
MFCD00005875
Beilstein/REAXYS Number:
61698
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Product Name

Bromophenol Blue, titration: suitable

Quality Level

200

agency

suitable for SM 2310, suitable for SM 2320

form

powder

technique(s)

titration: suitable

color

faint beige to very dark beige, faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

solubility

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000545643
0000545643
SHBT2989
0000460622
0000460622

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