G5154
Glasgow Minimum Essential Medium
With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
BHK medium, GMEM, Glasgow′s MEM
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About This Item
Product Name
Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.
Application
Glasgow Minimum Essential Medium has been used to culture:
- Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
- murine E14 Tg2a embryonic stem (mES) cells
- transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins
Preparation Note
Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Marieke Aarts et al.
Genes & development, 31(20), 2085-2098 (2017-11-16)
Expression of the transcription factors OCT4, SOX2, KLF4, and cMYC (OSKM) reprograms somatic cells into induced pluripotent stem cells (iPSCs). Reprogramming is a slow and inefficient process, suggesting the presence of safeguarding mechanisms that counteract cell fate conversion. One such
Sacha Robert et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known.