H1136
Hyaluronidase from Streptomyces hyalurolyticus
Synonym(s):
Hyaluronate Lyase from Streptomyces hyalurolyticus
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-430-8
MDL number:
Biological source:
Streptomyces sp. (S. hyalurolyticus)
biological source
Streptomyces sp. (S. hyalurolyticus)
conjugate
conjugate (Glucosaminoglycan)
form
film (Clear)
packaging
ampule of 300 × units
storage temp.
−20°C
Quality Level
Related Categories
General description
Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.
Application
Hyaluronidase from Streptomyces hyalurolyticus has been used:
- in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
- as a component of digestion solution for the derivation of germline stem cells from testicular tissue
Biochem/physiol Actions
Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Crystal structure of hyaluronidase, a major allergen of bee venom
Markovic H, et al.
Structure, 8(10), 1025-1035 (2000)
Connor C Gains et al.
Frontiers in bioengineering and biotechnology, 8, 700-700 (2020-08-09)
The Achilles tendon (AT) is comprised of three distinct sub-tendons bound together by the inter-subtendon matrix (ISTM). The interactions between sub-tendons will have important implications for AT function. The aim of this study was to investigate the extent to which
Wen Min Lau et al.
Cancer research, 74(9), 2630-2641 (2014-03-13)
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In
Tímea Váradi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), 567-575 (2012-05-09)
Although trastuzumab is an efficient drug, primary and acquired resistance is a challenging problem. The authors have previously shown in mouse xenograft experiments that masking ErbB2 by hyaluronan leads to diminished binding of the antibody and consequent resistance. In the
Caitlin E Henderson-Toth et al.
Developmental biology, 369(2), 330-339 (2012-07-24)
The glycocalyx, and the thicker endothelial surface layer (ESL), are necessary both for endothelial barrier function and for sensing mechanical forces in the adult. The goal of this study is to use a combination of imaging techniques to establish when
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