T7039
Transforming Growth Factor-β1 human
TGF-β1, recombinant, expressed in CHO cells, powder, suitable for cell culture
Synonym(s):
hTGF-β1, TGF-β1
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About This Item
Biological source:
human
Recombinant:
expressed in CHO cells
Assay:
≥95% (SDS-PAGE)
Form:
powder
Mol wt:
dimer (12.8/25.6 kDa (112/224 aa))
Impurities:
≤0.1 EU/μg (LAL test)
biological source
human
recombinant
expressed in CHO cells
form
powder
Quality Level
assay
≥95% (SDS-PAGE)
potency
≤0.5 ng/mL ED50
mol wt
dimer (12.8/25.6 kDa (112/224 aa))
packaging
pkg of 2 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤0.1 EU/μg (LAL test)
UniProt accession no.
storage temp.
−20°C
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General description
Tgfb1 (transforming growth factor-β1 human) is a 25kDa peptide encoded by the gene mapped to human chromosome 19q13.2. TGF-β1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3.
Application
Transforming Growth Factor-β1 human has been used in
- reporter gene assay.
- in vitro transfection.
- to study the effect of transforming growth factor-β1 (TGF-β1) on gene array analysis of renal cells.
- to study the association of TGF-β1 with shear stress-dependent upregulation of tissue inhibitor of metalloproteinase-1 in microvascular endothelial cells.
- to study the interaction between TGF-β1 activation and myofibroblast formation.
Biochem/physiol Actions
TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, tissue growth, differentiation, and other functions in a wide range of cell types. TGF-β1 plays a fundamental role in differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. All cells possess a specific TGF-β1 receptor. Both transformed and non-neoplastic tissues release transforming growth factors.The multi-modal nature of TGF-β1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
Physical form
Lyophilized from a 0.2 µm filtered solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio
Preparation Note
Reconstitution: Sterile 10 mM HCl at 0.1 mg/mL
Analysis Note
The biological activity of TGF-β1 is measured in culture by inhibition of mouse IL-4-dependent proliferation of mouse HT-2 cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Matrix metalloproteinase inhibition delays wound healing and blocks latent transforming growth factor-β1 promoted myofibroblast formation and function
Mirastschijski U, et al.
Wound Repair and Regeneration, 18(2), 223-234 (2010)
Interplay between TGF-β signaling and receptor tyrosine kinases in tumor development.
Shi Q and Chen YG
Science China: Life Sciences, 60(10), 1133-1141 (2017)
Differential Effects of Transforming Growth Factor-β1 on Cellular Proliferation in the Developing Prostate
Tomlinson DC, et al.
Endocrinology, 145, 4292-4300 (2004)
Tibor Kempf et al.
Clinical chemistry, 53(2), 284-291 (2006-12-23)
Growth-differentiation factor 15 (GDF15) is a member of the transforming growth factor beta (TGF-beta) cytokine superfamily. There has been increasing interest in using circulating GDF15 as a biomarker in patients, for example those with cardiovascular disease. We developed an IRMA
Louis A Saddic et al.
Cardiovascular diabetology, 16(1), 87-87 (2017-07-09)
Omentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has
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