535893
5-Methyluridine
97%
Synonym(s):
Ribothymidine
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About This Item
Empirical Formula (Hill Notation):
C10H14N2O6
CAS Number:
Molecular Weight:
258.23
NACRES:
NA.22
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
215-973-9
MDL number:
Assay:
97%
assay
97%
mp
183-184 °C (lit.)
SMILES string
CC1=CN([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C(=O)NC1=O
InChI
1S/C10H14N2O6/c1-4-2-12(10(17)11-8(4)16)9-7(15)6(14)5(3-13)18-9/h2,5-7,9,13-15H,3H2,1H3,(H,11,16,17)/t5-,6-,7-,9-/m1/s1
InChI key
DWRXFEITVBNRMK-JXOAFFINSA-N
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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H F Becker et al.
Journal of molecular biology, 274(4), 505-518 (1998-01-07)
Almost all transfer RNA molecules sequenced so far contain two universal modified nucleosides at positions 54 and 55, respectively: ribothymidine (T54) and pseudouridine (psi 55). To identify the tRNA elements recognized by tRNA:m5uridine-54 methyltransferase and tRNA:pseudouridine-55 synthase from the yeast
Benoit Desmolaize et al.
Nucleic acids research, 39(21), 9368-9375 (2011-08-10)
Methyltransferases that use S-adenosylmethionine (AdoMet) as a cofactor to catalyse 5-methyl uridine (m(5)U) formation in tRNAs and rRNAs are widespread in Bacteria and Eukaryota, and are also found in certain Archaea. These enzymes belong to the COG2265 cluster, and the
Gregory E R Gordon et al.
Journal of biotechnology, 151(1), 108-113 (2010-11-30)
This paper describes a high yielding coupled enzymatic reaction using Bacillus halodurans purine nucleoside phosphorylase (PNP) and E. coli uridine phosphorylase (UP) for synthesis of 5-methyluridine (5-MU) by transglycosylation. Key parameters such as reaction temperature, pH, reactant loading, reactor configuration