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N8010

4-Nitrophenyl 4-guanidinobenzoate hydrochloride

Name: 4-Nitrophenyl 4-guanidinobenzoate hydrochloride
Brand: SIGMA

protease substrate, ≥95.0% (TLC), powder

Synonym(s):

4-Guanidinobenzoic acid 4-nitrophenylester hydrochloride, NPGB, pNPGB

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₂N₄O₄ · HCl

CAS Number:

19135-17-2

Molecular Weight:

336.73

NACRES:

NA.32

PubChem Substance ID:

24897847

UNSPSC Code:

12352204

EC Number:

242-831-3

MDL number:

MFCD00037010

Beilstein/REAXYS Number:

8024554

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Properties

Product Name

4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate

Quality Segment

200

assay

≥95.0% (TLC)

form

powder

solubility

formic acid: soluble 49.00-51.00 mg/mL

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)Nc1ccc(cc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H

InChI key

PKSBDZOBYIKNGY-UHFFFAOYSA-N

Description

General description

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.

Application

4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:

as a substrate for trypsin for active site titration experiments
for pre-treating of mosquito eggs in the interplasmid transposition assay
as a component of isotonic buffer to moisten filter paper for mosquito embryo collection

Biochem/physiol Actions

4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.

pictograms

GHS05

signalword

Danger

hcodes

H318

pcodes

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c