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Merck

T8657

Trichloroacetic acid

suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

Synonym(s):

TCA

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
EC Number:
200-927-2
Beilstein/REAXYS Number:
970119
MDL number:

Product Name

Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

grade

electrophoresis grade

vapor density

<1 (vs air)

vapor pressure

1 mmHg ( 51 °C)

assay

≥99%

form

powder or flakes (or crystals)

technique(s)

electrophoresis: suitable

turbidity

≤3 NTU (1 g/ml)

refractive index

n20/D 1.62 (lit.)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

water: 1 g/mL, clear, colorless

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤0.001%
nitrate (NO3-): ≤0.002%
phosphate (PO43-): ≤5 ppm
sulfate (SO42-): ≤0.02%

cation traces

Fe: ≤0.0005%
heavy metals (as Pb): ≤0.002%

suitability

suitable for fixing solution (for IEF and PAGE gels)

application(s)

cell analysis

storage temp.

2-8°C

Quality Level

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Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.
Trichloroacetic acid has been used:
  • for hydrolysis of DNA isolated from the cell
  • for precipitation of glutathione isolated from seminal plasma
  • as a fixative in microscopy

General description

Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material. It is a novel reagent used in clinical laboratories for deproteinization of body fluids. In addition, TCA is also used in the treatment of genital warts and acne scars.

pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Focal treatment of acne scars with trichloroacetic acid: chemical reconstruction of skin scars method
Lee J, et al.
Dermatologic Surgery : Official Publication For American Society For Dermatologic Surgery [Et Al.], 28(11), 1017-1021 (2002)
Quantitation of DNA fragmentation with diphenylamine
Boraschi D and Maurizi G
Apoptosis-a laboratory manual of experimental methods, 153-161 (1998)
Teratogenic activity of trichloroacetic acid in the rat
Smith MK, et al.
Teratology, 40(5), 445-451 (1989)
Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid
Meulemans O
Clinica Chimica Acta; International Journal of Clinical Chemistry, 5(5), 757-761 (1960)
Atmospheric pressure pulsed plasma induces cell death in photosynthetic organs via intracellularly generated ROS
Seol Y, et al.
Scientific reports, 7(1), 589-589 (2017)

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