Anti-ASF1a Antibody

ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA inside PML bodies of cells approaching senescence after p53/p21 activation.

~23 kDa observed

GST-tagged recombinant protein corresponding to human ASF1a.

Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Banumathy, G, et al. (2009). Mol Cell Biol. 29(3):758–770.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected ASF1a on 10 µg of HeLa cell lysate.

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