Anti-IL18R1 Antibody, clone H44

Interleukin-18 receptor 1 (UniProt: ; also known as EC:3.2.2.6, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein (IL-1Rrp; IL1R-rp), Interleukin-18 receptor alpha, IL-18R-alpha, IL-18Ralpha, CD218a) is encoded by the IL18R1 (also known as IL1RRP) gene (Gene ID: 8809) in human. Interleukin-18 receptor 1 (IL-18R1) is a crucial component of the immune system, mediating the activity of interleukin-18 (IL-18), a pro-inflammatory cytokine that belongs to the IL-1 family. IL-18R1 forms a heterodimeric receptor complex with IL-18 receptor β (IL-18Rβ). IL-18R1 is composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular Toll/interleukin-1 receptor (TIR) domain. Crystal structures of the receptor complex demonstrate specific ligand-binding interactions governed by surface charge complementarity, ensuring specificity in IL-18 signaling. Upon IL-18 binding, the receptor complex initiates intracellular signal transduction via the MyD88-dependent pathway, leading to activation of NF- B and MAPK pathways, and subsequent production of interferon- (IFN- ). The receptor is expressed predominantly in immune cells such as T cells, natural killer (NK) cells, and macrophages, as well as non-immune cells like neurons and epithelial cells. The receptor plays a pivotal role in inflammatory responses, autoimmune diseases, and infectious diseases by driving cytokine production. Dysregulation of IL-18R1 signaling has been implicated in a range of diseases, including rheumatoid arthritis, Crohn s disease, and certain cancers. Downregulation of IL-18R1 has been associated with enhanced cancer cell proliferation and migration, leading to poor clinical outcomes. IL-18R1 has also been implicated in neuroinflammation and metabolic disorders. (Ref.: Tsutsumi, N., et al., (2014). Nat Commun. 5, 5340)

P815 mast cells transfected with IL18R1.

Quality Control Testing

Evaluated by ELISA with recombinant IL18R1 protein fragment.

Enzyme Immunoassay: A representative lot of this antibody detected recombinant IL18R1 protein fragment in ELISA application.

Flow Cytometry Analysis: A representative lot detected IL18R1 in Flow Cytometry application (Kitasato, Y., et al. (2004). Am J Respir Cell Mol Biol. 31(6):619-25).

Western Blotting Analysis: A representative lot detected IL18R1 in Western Blotting application (Kitasato, Y., et al. (2004). Am J Respir Cell Mol Biol. 31(6):619-25).

Immunohistochemistry Applications: A representative lot detected IL18R1 in Immunohistochemistry applications (Kitasato, Y., et al. (2004). Am J Respir Cell Mol Biol. 31(6):619-25).

Neutralizing Assay: A representative lot had neutralizing activity against recombinant human IL-18 in Neutralizing applications (Kitasato, Y., et al. (2004). Am J Respir Cell Mol Biol. 31(6):619-25).

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone H44 is a mouse recombinant monoclonal antibody that detects IL18R1.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stable for 1 year at -10°C to -25°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.