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D9542

DAPI

Name: DAPI
Brand: SIGMA

for nucleic acid staining

Synonym(s):

4′,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₅N₅ · 2HCl

CAS Number:

28718-90-3

Molecular Weight:

350.25

UNSPSC Code:

12352200

NACRES:

NA.52

PubChem Substance ID:

24894305

EC Number:

249-186-7

Beilstein/REAXYS Number:

4894417

MDL number:

MFCD00012681

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Properties

grade

Molecular Biology

Quality Segment

300

assay

≥98% (HPLC)

form

powder

technique(s)

transfection: suitable

solubility

H₂O: 20 mg/mL, PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H₂O at 1 mM

fluorescence

λ_ex 340 nm; λ_em 488 nm (nur DAPI), λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

Application

Suitable for

DNA staining in agarose gels
analysis of changes in DNA during apoptosis
detection of mycoplasma
photofootprinting of DNA
immunofluorescent staining of cells

DAPI has been used:-

in rapid monitoring of microbial contamination
in chromosomal banding technique
in detection of apoptotic cells
in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
to stain mature pollen grains(0.5 mg/ml)

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Protect from light.

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H315,H317,H335

Precautionary Statements

P261 - P264 - P271 - P272 - P280 - P302 + P352

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)