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N2765

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
NACRES:
NA.83
PubChem Substance ID:
24897592
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
MFCD00066288
Beilstein/REAXYS Number:
5324661
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Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

form

tablet

Quality Level

300

solubility

water, high purity: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
  • as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
  • in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample

Preparation Note

Contains 20 mg substrate per tablet

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000518239
0000518239
0000486044
0000512549
0000496018

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Amanda L Miller et al.
PLoS pathogens, 16(7), e1008591-e1008591 (2020-07-10)
Reactive arthritis, an autoimmune disorder, occurs following gastrointestinal infection with invasive enteric pathogens, such as Salmonella enterica. Curli, an extracellular, bacterial amyloid with cross beta-sheet structure can trigger inflammatory responses by stimulating pattern recognition receptors. Here we show that S.
Marcus J Hines et al.
Cell reports, 33(9), 108436-108436 (2020-12-03)
The phosphatidylinositol 3-kinase (PI3K) signaling cascade downstream of the B cell receptor (BCR) signalosome is essential for B cell maturation. Proper signaling strength is maintained through the PI3K negative regulator phosphatase and tensin homolog (PTEN). Although a role for microRNA
Mihaela Gadjeva et al.
Journal of immunology (Baltimore, Md. : 1950), 184(1), 296-302 (2009-12-02)
The inflammatory response to Pseudomonas aeruginosa is not properly regulated in the lungs of patients with cystic fibrosis (CF). In the lung epithelium of individuals with wild-type CF transmembrane conductance regulator, lipid rafts containing CF transmembrane conductance regulator are rapidly
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