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S7388

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide

Name: N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide
Brand: SIGMA

Subtilisin substrate

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide, N-Succinyl-Ala-Ala-Pro-Phe-pNA

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About This Item

Empirical Formula (Hill Notation):

C₃₀H₃₆N₆O₉

CAS Number:

70967-97-4

Molecular Weight:

624.64

NACRES:

NA.32

PubChem Substance ID:

24899742

UNSPSC Code:

12352200

MDL number:

MFCD00077161

Beilstein/REAXYS Number:

4289896

Quality level:

300

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Properties

Product Name

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide,

Quality Level

300

storage temp.

−20°C

SMILES string

[H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(O)=O)C(=O)N[C@@H](Cc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C30H36N6O9/c1-18(31-25(37)14-15-26(38)39)27(40)32-19(2)30(43)35-16-6-9-24(35)29(42)34-23(17-20-7-4-3-5-8-20)28(41)33-21-10-12-22(13-11-21)36(44)45/h3-5,7-8,10-13,18-19,23-24H,6,9,14-17H2,1-2H3,(H,31,37)(H,32,40)(H,33,41)(H,34,42)(H,38,39)/t18-,19-,23-,24-/m0/s1

InChI key

LKDMKWNDBAVNQZ-WJNSRDFLSA-N

Gene Information

human ... FKBP10(60681)

Description

General description

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a colorimetric substrate for chymotrypsin, human leukocyte cathepsin G and peptidyl prolyl isomerase.

Application

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used:

as a substrate for cathepsin G assay in bone marrow lysates.
in proteolytic activity assay of serine protease subtilisin carlsberg (SC).
as a substrate for neutrophil elastase, trypsin and chymotrypsin in protease assay.

Biochem/physiol Actions

N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a substrate for subtilisin and proteases.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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