159220
Diethyl pyrocarbonate
96% (NT)
Synonym(s):
DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
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About This Item
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
216-542-8
Beilstein/REAXYS Number:
637031
MDL number:
Assay:
96% (NT)
Form:
liquid
Quality Level
assay
96% (NT)
form
liquid
refractive index
n20/D 1.398 (lit.)
bp
93-94 °C/18 mmHg (lit.)
density
1.101 g/mL at 25 °C (lit.)
functional group
carbonate
shipped in
wet ice
storage temp.
2-8°C
SMILES string
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
General description
Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.
Application
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Diethyl pyrocarbonate was used as:
- nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
- an antimicrobial additive
- ribonuclease inhibitor
- histidine residue modifier
- reagent for conversion of imines to carbamates
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
156.2 °F - closed cup
flash_point_c
69 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Heather L Walsh et al.
PloS one, 15(8), e0236104-e0236104 (2020-08-11)
There is an increasing emphasis on effects-based monitoring to document responses associated with exposure to complex mixtures of chemicals, climate change, pathogens, parasites and other environmental stressors in fish populations. For decades aquatic monitoring programs have included the collection of
A mechanism of the irreversible inactivation of bovine pancreatic ribonuclease by diethylpyrocarbonate. A general reaction of diethylpyrocarbonate . A general reaction of diethylpyrocarbonate with proteins.
B Wolf et al.
European journal of biochemistry, 13(3), 519-525 (1970-04-01)
Isolation of plant RNA.
R Hodge
Methods in molecular biology (Clifton, N.J.), 28, 37-40 (1994-01-01)