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Merck

D218200

5,5′-Dithiobis(2-nitrobenzoic acid)

ReagentPlus®, 99%

Synonym(s):

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

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About This Item

Linear Formula:
[-SC6H3(NO2)CO2H]2
CAS Number:
69-78-3
Molecular Weight:
396.35
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
24893651
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
MFCD00007140
Assay:
99%
Form:
powder

Key Documents

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InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

product line

ReagentPlus®

assay

99%

form

powder

mp

240-245 °C (dec.) (lit.)

Quality Level

200

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General description

DTNB (Ellman’s reagent) is commonly used for quantification of sulfhydryl (thiol) groups in proteins and biomolecules.

Application

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK8675
STBJ6791
STBG6026V
STBG1024V
STBD3094V

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Pork proteins oxidative modifications under the influence of varied time-temperature thermal treatments: A chemical and redox proteomics assessment.
Mitra B, et al.
Meat Science, 140, 134-144 (2018)
Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid
W Chen, et al.
Journal of Pharmaceutical and Biomedical Analysis, 48, 1375-1380 (2008)
Atef M K Nassar et al.
Molecules (Basel, Switzerland), 25(11) (2020-06-11)
Sumithion (Fenitrothion) (SUM) is an organophosphorus insecticide used to combat a wide variety of plant pests. Exposure to SUM causes significant toxicity to the brain, liver, kidney, and reproductive organs through, for example, binding to DNA, and it induces DNA
Anne Bertling et al.
Arteriosclerosis, thrombosis, and vascular biology, 32(8), 1979-1990 (2012-04-28)
Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein
Malgorzata Boczkowska et al.
Structure (London, England : 1993), 16(5), 695-704 (2008-05-09)
Previous structures of Arp2/3 complex, determined in the absence of a nucleation-promoting factor and actin, reveal its inactive conformation. The study of the activated structure has been hampered by uncontrollable polymerization. We have engineered a stable activated complex consisting of
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