N21804
3-Nitrophenylhydrazine hydrochloride
98%
Synonym(s):
(3-Nitrophenyl)hydrazine hydrochloride, 1-(3-Nitrophenyl)hydrazine hydrochloride, 3-Nitrophenylhydrazine monohydrochloride, m-Nitrophenylhydrazine hydrochloride
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About This Item
Linear Formula:
O2NC6H4NHNH2 · HCl
CAS Number:
Molecular Weight:
189.60
NACRES:
NA.22
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
211-270-6
MDL number:
Assay:
98%
Form:
powder
Quality Level
assay
98%
form
powder
mp
210 °C (dec.) (lit.)
SMILES string
Cl.NNc1cccc(c1)[N+]([O-])=O
InChI
1S/C6H7N3O2.ClH/c7-8-5-2-1-3-6(4-5)9(10)11;/h1-4,8H,7H2;1H
InChI key
BKOYKMLGFFASBG-UHFFFAOYSA-N
General description
3-Nitrophenylhydrazine (3-NPH) is a derivatizing reagent used for the derivatization of carboxyl- and carbonyl-containing metabolites. This compound is also used as a high-efficiency chemical isotope-labeling reagent. Additionally, 3-nitrophenylhydrazine undergoes a condensation reaction with substituted benzaldehydes resulting in the formation of a series of ten substituted (E)-1-benzylidene-2-(3-nitrophenyl) hydrazine compounds.
Application
<ul>
<li><strong>Derivatization agent in analytical chemistry:</strong> 3-Nitrophenylhydrazine hydrochloride is employed as a derivatization agent for the determination of gamma-Hydroxybutyric acid in biological samples infected with Herpes Simplex Virus-Type 1, utilizing LC-MRM-MS techniques, providing enhanced detection capabilities in forensic and clinical toxicology (Osinaga et al., 2022).</li>
<li><strong>Residual analysis in pharmaceutical formulations:</strong> This compound is also used for the quantitative analysis of residual 4-nitrobenzaldehyde in chloramphenicol and its pharmaceutical formulations, employing HPLC with UV/Vis detection after derivatization with 3-nitrophenylhydrazine, offering a reliable method for ensuring the purity and safety of pharmaceutical products (Luo et al., 2018).</li>
<li><strong>Forensic application in decomposition analysis:</strong> Used to derivatized the blood samples collected at the time of forensic autopsy (Matoba et al., 2022).</li>
</ul>
<li><strong>Derivatization agent in analytical chemistry:</strong> 3-Nitrophenylhydrazine hydrochloride is employed as a derivatization agent for the determination of gamma-Hydroxybutyric acid in biological samples infected with Herpes Simplex Virus-Type 1, utilizing LC-MRM-MS techniques, providing enhanced detection capabilities in forensic and clinical toxicology (Osinaga et al., 2022).</li>
<li><strong>Residual analysis in pharmaceutical formulations:</strong> This compound is also used for the quantitative analysis of residual 4-nitrobenzaldehyde in chloramphenicol and its pharmaceutical formulations, employing HPLC with UV/Vis detection after derivatization with 3-nitrophenylhydrazine, offering a reliable method for ensuring the purity and safety of pharmaceutical products (Luo et al., 2018).</li>
<li><strong>Forensic application in decomposition analysis:</strong> Used to derivatized the blood samples collected at the time of forensic autopsy (Matoba et al., 2022).</li>
</ul>
signalword
Warning
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Lin-Xiu Guo et al.
Molecules (Basel, Switzerland), 25(23) (2020-12-10)
Short-chain fatty acids (SCFAs) are the main microbial fermentation products from dietary fibers in the colon, and it has been speculated that they play a key role in keeping healthy in the whole-body. However, differences in SCFAs concentration in the
François-Xavier Danlos et al.
Cell death & disease, 12(3), 258-258 (2021-03-13)
The circulating metabolome provides a snapshot of the physiological state of the organism responding to pathogenic challenges. Here we report alterations in the plasma metabolome reflecting the clinical presentation of COVID-19 patients with mild (ambulatory) diseases, moderate disease (radiologically confirmed
Derivatization of N-acyl glycines by 3-nitrophenylhydrazine for targeted metabolomics analysis and their application to the study of diabetes progression in mice
Xiang, Li and Ru, et al.
Analytical Chemistry, 95, 2183-2191 (2023)