05-384
Anti-α-Actinin Antibody, clone AT6/172
clone AT6/172, Upstate®, from mouse
Synonym(s):
Anti-BDPLT15
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AT6/172, monoclonal
Application:
ICC, WB
Citations:
73
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AT6/172, monoclonal
species reactivity
rabbit, human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ACTN1(87)
General description
100 kDa
Immunogen
Human α-actinin
Application
Immunoprecipitation is not recommended
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.
Biochem/physiol Actions
Recognizes α-actinin.
Physical form
Format: Purified
Please see product datasheet for lot-specific formulation.
Protein A purified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated in immunoblot on human A431 cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jing Zong et al.
Laboratory investigation; a journal of technical methods and pathology, 93(10), 1128-1136 (2013-08-21)
Nucleotide-binding oligomerization domain-2 (NOD2, also designated CARD15), a member of the NOD-leucine-rich repeat (LRR) protein family (also called the CATERPILLAR family), is upregulated in atheroma lesions and has an important role in regulating the intracellular recognition of bacterial components by
Requirement of phosphatidylinositol 4,5-bisphosphate for alpha-actinin function
Fukami, K, et al
Nature, 359, 150-152 (1992)
Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy.
Bian, Z; Liao, H; Zhang, Y; Wu, Q; Zhou, H; Yang, Z; Fu, J; Wang, T; Yan, L; Shen, D; Li, H; Tang, Q
Testing null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-384 | 04053252732645 |