16-101
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
clone 4G10®, Upstate®, from mouse
Synonym(s):
4G10 Phosphotyrosine Antibody, Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4G10®, monoclonal
Species reactivity:
vertebrates
Application:
IP
Technique(s):
immunoprecipitation (IP): suitable
Citations:
110
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PID1(55022)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Analysis Note
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Application
Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Suitable for immunoprecipitation and affinity purification.
Biochem/physiol Actions
Recognizes tyrosine-phosphorylated proteins from all species.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Format: Gel Immobilized
General description
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen
Phosphotyramine-KLH.
Physical form
Protein A purified
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% preservative, for a total volume of 2 mL. Liquid suspension.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Heparin binding and oligomerization of hepatocyte growth factor/scatter factor isoforms. Heparan sulfate glycosaminoglycan requirement for Met binding and signaling.
Sakata, H, et al.
The Journal of Biological Chemistry, 272, 9457-9463 (1997)
Phosphorylation site dynamics of early T-cell receptor signaling.
Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
Testing null
Growth hormone induction of hepatic serine protease inhibitor 2.1 transcription is mediated by a Stat5-related factor binding synergistically to two gamma-activated sites
Bergad, P. L., et al
The Journal of Biological Chemistry, 270, 24903-24910 (1995)
Erik J Henriksen et al.
Metabolism: clinical and experimental, 56(7), 931-938 (2007-06-16)
Overactivity of glycogen synthase kinase 3 (GSK-3) is associated with insulin resistance of skeletal muscle glucose transport in prediabetic and type 2 diabetic rodent models. However, limited information is available concerning the potential molecular mechanisms underlying the role GSK-3 plays
Endogenous reactive oxygen intermediates activate tyrosine kinases in human neutrophils
Brumell, J. H., et al
The Journal of Biological Chemistry, 271, 1455-1461 (1996)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 16-101 | 04053252514951 |
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