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Merck

EZGLP1T

Multi Species GLP-1 ELISA Kit

measures total GLP-1 levels in 20 µl - 50 µl serum, plasma or cell culture supernatent

Synonym(s):

Glucagon-like peptide-1

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Gene information:
human ... GCG(2641)
Input:
sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum
Species reactivity:
human, mouse, rat
Storage temp.:
2-8°C
Shipped in:
wet ice

Key Documents

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Product Name

Multi Species GLP-1 Total ELISA, EZGLP1T-36K, This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.

species reactivity

human, mouse, rat

packaging

kit of 1 × 96 wells

parameter

20—50 μL sample volume (4hr assay)

assay range

linearity: 90-132%, recovery range: 90-110%, sensitivity: 1.5 pM
(MinDC), standard curve range: 4.1-1000 pM, inter-assay cv: <12%
intra-assay cv: <5%

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

NCBI accession no.

NM_002054.2

UniProt accession no.

P01275

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... GCG(2641)

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General description

Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.

Application

Research Category
Metabolism
This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Used to detect/quantify: Glucagon Like Peptide-1

Biochem/physiol Actions

Cross Reactivty
GLP-1 is conserved in almost all mammals.
The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.

Disclaimer

For research use only. Not for use in diagnostic procedures.

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Certificates of Analysis (COA)

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Yasutaka Takeda et al.
Physiological reports, 8(11), e14469-e14469 (2020-05-31)
The short-form glucose-dependent insulinotropic polypeptide (GIP) (1-30) is released from islet alpha cells and promotes insulin secretion in a paracrine manner in vitro. However, it is not well elucidated how GIP (1-30) is involved in glucose metabolism in vivo, since
Lindsey M Rings et al.
Journal of veterinary internal medicine, 33(6), 2752-2764 (2019-10-31)
The enteroinsular axis (EIA) comprises intestinal factors (incretins) that stimulate insulin release after PO ingestion of nutrients. Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are the main incretins. The EIA has not been investigated in healthy neonatal foals but

Global Trade Item Number

SKUGTIN
EZGLP1T-36BK04053252436888
EZGLP1T-36K04053252749148

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