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Merck

HSTCMAG-28SK

MILLIPLEX® Human High Sensitivity T Cell Panel

Configurable 96-well Human High Sensitivity T Cell 21-Plex Panel

Synonym(s):

Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

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species reactivity

human

Quality Level

200

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

accuracy: 98-107%, sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.45-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

serum
cell culture supernatant

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 21 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • High-Sensitivity Detection: Enables the detection of low-abundance T cell cytokines, providing critical insights into subtle immune responses and enhancing understanding of disease mechanisms.
  • Simultaneous Quantification of Multiple Analytes: Capable of measuring a variety of T cell-related cytokines and chemokines in a single assay run, including IL-1β, IL-2, IL-6, IL-10, TNFα, and others, saving time and resources.
  • Versatile Sample Compatibility: Offers flexibility in sample types, making the assay applicable to a wide range of research settings and experimental designs.
  • Enhanced Understanding of Immune Responses: Contributes to a deeper understanding of the immune system′s complex interactions, particularly in the context of inflammatory diseases and other clinical conditions.
  • Cardiovascular Risk Profiling: Analyze IFN-γ and MIP-1α, associated with atherosclerotic plaque instability to identify biomarkers for cardiovascular event prediction or therapeutic monitoring.
  • Subpanel Flexibility: Select subsets of analytes (e.g., IL-2, IL-4, IL-5) for focused hypotheses, reducing costs and streamline workflows for targeted immune monitoring.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anne H Krog et al.
Vascular health and risk management, 12, 371-378 (2016-10-08)
Minimally invasive surgical techniques have been shown to reduce the inflammatory response related to a surgical procedure. The main objective of our study was to measure the inflammatory response in patients undergoing a totally laparoscopic versus open aortobifemoral bypass surgery.
Matteo Ravaioli et al.
Scientific reports, 10(1), 6063-6063 (2020-04-10)
With the aim to explore innovative tools for organ preservation, especially in marginal organs, we hereby describe a clinical trial of ex-vivo hypothermic oxygenated perfusion (HOPE) in the field of liver (LT) and kidney transplantation (KT) from Extended Criteria Donors
Malcolm Sanderson et al.
Sports (Basel, Switzerland), 8(12) (2020-12-05)
Swimming is a popular youth sport that is considered beneficial for cardiovascular fitness. However, the potential inflammatory outcomes of high intensity swimming in younger swimmers are unclear, as is the response of irisin, a myokine released during exercise with anti-inflammatory
Bruna M Ascoli et al.
International journal of bipolar disorders, 7(1), 13-13 (2019-06-04)
Innate immune system dysfunction has been recognized as an important element in the pathophysiology of bipolar disorder (BD). We aimed to investigate whether there are differences in the response of macrophages derived from patients in the early stages and late
B Karshikoff et al.
Brain, behavior, and immunity, 57, 38-46 (2016-04-09)
Low-grade systemic inflammation has been implicated in chronic pain, as well as in comorbid diseases like depression and fatigue. We have previously shown that women's pain perception and regulation is more affected by systemic inflammation than that of men. Here
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Global Trade Item Number

SKUGTIN
HSTCSF-28SK-03C04065267242835
HSTCSF-28SK-04C04065267242842
HSTCSF-28SK-09C04065267242897
HSTCSF-28SK-01C04065267242811
HSTCSF-28SK-1004065267242422
HSTCSF-28SK-13C04065267242934
HSTCSF-28SK-1804065267242507
HSTCSF-28SK-1304065267242453
HSTCSF-28SK-1504065267242477
HSTCSF-28SK-2004065267242521
HSTCSF-28SK-1904065267242514
HSTCSF-28SK-20C04065267243009

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