MAB5272
Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
clone 324, Chemicon®, from rat
Synonym(s):
CD171, N-CAM L1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
324, monoclonal
Application:
ICC, IHC, WB
Citations:
74
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
324, monoclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... L1CAM(3897), VCAM1(7412)
General description
120-140 kDa
Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
Immunogen
Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
Application
This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
Western blotting:
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
20 μg/mL was used on a previous lot.
Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.
Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
Physical form
Format: Purified
Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
Testing null
ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex.
Li, Q; Zhang, Z; Li, Z; Zhou, M; Liu, B; Pan, L; Ma, Z; Zheng, Y
Testing null
Evidence that descending cortical axons are essential for thalamocortical axons to cross the pallial-subpallial boundary in the embryonic forebrain.
Chen, Y; Magnani, D; Theil, T; Pratt, T; Price, DJ
Testing null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB5272 | 04053252502118 |