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Merck

G6639

Guanidine thiocyanate

≥97% (titration)

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
UNSPSC Code:
12352209
NACRES:
NA.55
PubChem Substance ID:
24895264
EC Number:
209-812-1
Beilstein/REAXYS Number:
3563461
MDL number:
MFCD00013027

Key Documents

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Product Name

Guanidine thiocyanate, ≥97% (titration)

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

SMILES string

SC#N.NC(N)=N

biological source

synthetic

assay

≥97% (titration)

form

powder or crystals

technique(s)

RNA extraction: suitable

color

white to off-white

mp

120-122 °C (lit.)

solubility

water: 4 M, clear, colorless

anion traces

chloride (Cl-): ≤2.5%

Quality Level

200

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Application

Chaotropic agent and strong denaturant; solubilizes cells.
Guanidine thiocyanate has been used:
  • as a denaturing agent to study its effects on surface activity of globular protein
  • for the extraction of total RNA from primary hepatocytes
  • as a component of the incubating buffer for extraction of DNA from human bone
  • as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
  • as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF4727V
WXBF6711V
0000100363
0000098775
0000114932

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Emilia Bramanti et al.
Analytical biochemistry, 351(1), 100-113 (2006-01-28)
In this article, a multidimensional dynamic surface tension detector (DSTD), in a parallel configuration with a UV-visible diode array absorbance detector, is presented in a novel flow injection analysis (FIA) application to study the effects of chemical denaturants urea, guanidinium
Julian R Peat et al.
F1000Research, 6, 526-526 (2017-06-06)
Methylation of CG dinucleotides constitutes a critical system of epigenetic memory in bony vertebrates, where it modulates gene expression and suppresses transposon activity. The genomes of studied vertebrates are pervasively hypermethylated, with the exception of regulatory elements such as transcription
Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique
Soni N
PharmaTutor, 4, 20-22 (2016)
DNA fromMycobacterium tuberculosisIdentified in Mediaeval Human Skeletal Remains Using Polymerase Chain Reaction
Taylor M G, et al.
Journal of Archaeological Science, 23, 789-798 (1996)
Victoire Cardot-Ruffino et al.
Scientific reports, 10(1), 3880-3880 (2020-03-05)
Transforming growth factor (TGFβ) is a secreted factor, which accumulates in tissues during many physio- and pathological processes such as embryonic development, wound healing, fibrosis and cancer. In order to analyze the effects of increased microenvironmental TGFβ concentration in vivo
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