N2125
Naphthol AS-BI phosphate
≥98% purity (HPLC), powder
Synonym(s):
7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate
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About This Item
Empirical Formula (Hill Notation):
C18H15BrNO6P
CAS Number:
Molecular Weight:
452.19
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
217-645-0
MDL number:
Beilstein/REAXYS Number:
6668526
Product Name
Naphthol AS-BI phosphate, Technical grade, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
technique(s)
microbe id | staining: suitable
color
white to light yellow
solubility
methanol: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O
InChI
1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
InChI key
HUXIAXQSTATULQ-UHFFFAOYSA-N
General description
Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.
Substrate for the histochemical demonstration of acid and alkaline phosphatase.
Application
Naphthol AS-BI phosphate is suitable to use:
- in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
- as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
- to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.
Biochem/physiol Actions
Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b.
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.
P J Stoward et al.
Histochemistry, 71(3), 405-431 (1981-01-01)
M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were