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Merck

N4754

Nigrosin water soluble

Powder

Synonym(s):

Acid black 2

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About This Item

CAS Number:
UNSPSC Code:
12171500
NACRES:
NA.47
Colour Index Number:
50420
MDL number:

Product Name

Nigrosin water soluble, For use as a biological stain

form

powder

solubility

water: 1 mg/mL, turbid

εmax

~250 at 575 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

Nigrosin has the following applications:
  • to stain live sperm
  • as a viable cell staining agent especially in stem cell biology
  • as a negative stain in place of India ink for spirochetes, bacteria, protozoa, and fungi
  • in Dorner′s spore stain.

General description

Nigrosin is an acidic dye used as a negative stain for visualization of the capsule in bacteriological material.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Experiments in Microbiology, Plant Pathology and Biotechnology (2003)
Broadband UV-Vis optical property measurement in layered turbid media.
Wang Q
SPIE Medical Imaging. International Society for Optics and Photonics, 2014 (2011)
Graff's Textbook of Routine Urinalysis and Body Fluids (2010)
Quanzeng Wang et al.
Biomedical optics express, 3(6), 1226-1240 (2012-06-29)
The ability to accurately measure layered biological tissue optical properties (OPs) may improve understanding of spectroscopic device performance and facilitate early cancer detection. Towards these goals, we have performed theoretical and experimental evaluations of an approach for broadband measurement of
Yanyu Zhao et al.
Nature communications, 11(1), 5355-5355 (2020-10-25)
Water and lipids are key participants in many biological processes, but there are few non-invasive methods that provide quantification of these components in vivo, and none that can isolate and quantify lipids in the blood. Here we develop a new

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