U5128
Urea
ACS reagent grade, ≤0.001% (Heavy Metals by ICP-OES, metals basis), 99.0-100.5%, solid
Synonym(s):
Carbamide, Carbonyldiamide
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About This Item
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12352100
EC Number:
200-315-5
MDL number:
Beilstein/REAXYS Number:
635724
Assay:
≤0.001% (Heavy Metals by ICP-OES, metals basis), 99.0-100.5%
Form:
solid
Product Name
Urea, ACS reagent, 99.0-100.5%
grade
ACS reagent
Quality Level
assay
≤0.001% (Heavy Metals by ICP-OES, metals basis), 99.0-100.5%
form
solid
impurities
≤0.01% Insoluble matter
ign. residue
≤0.01%
mp
132-135 °C (lit.)
solubility
H2O: soluble 480 g/L at 20 °C
density
1.335 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm, sulfate (SO42-): ≤0.001%
cation traces
Fe: ≤0.001%
functional group
amine
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
General description
Urea is a colourless organic compound, without any odour. It is easily soluble in water.
Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Urea has been used:
- to adjust the urea nitrogen concentration to a preferred range in blood reservoir for evaluating the dialyzer urea clearance
- to prepare standards to measure urea synthesis
- to prepare standards for blood urea nitrogen assay
Biochem/physiol Actions
Urea participates in the metabolism of nitrogenous substances. It serves as a nitrogen source, when used in fertilizers. Urea is used in manufacturing chemical compounds. It is widely used in the agricultural industry as a fertilizer as well as an animal feed additive, which is used as a nitrogen supplement in animal husbandry. Urea has adverse effects on seed germination, seedling growth and early plant growth in soil.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hemodialyzer mass transfer-area coefficients for urea increase at high dialysate flow rates
Leypoldt JK, et al.
Kidney International, 51(6), 2013-2017 (1997)
CHAPTER 16 - METHODS OF FEEDING UREA TO LIVESTOCK
Urea As a Protein Supplement, 321-327 (1967)
Urea: Synthesis, Properties and Uses (2012)