Y1500
YPD Agar
nonselective for Candida spp., Pichia spp., Saccharomyces spp., & Zygosaccharomyces spp.
Synonym(s):
Yeast Extract Peptone Dextrose Agar, YEPD Agar
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About This Item
UNSPSC Code:
50405901
NACRES:
NA.75
eCl@ss:
42040102
Product Name
YPD Agar,
form
powder
composition
Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L
suitability
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
Quality Level
application(s)
food and beverages
life science and biopharma
Application
YPD agar has been used as a culture media:
- to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
- for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
- for Zymoseptori triciti strains for virulence studies
General description
YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.
Preparation Note
1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ingrid A Edwards et al.
ACS infectious diseases, 3(12), 917-926 (2017-09-30)
Tachyplesin-1 (TP1; 1) is a cationic β-hairpin antimicrobial peptide with a membranolytic mechanism of action. While it possesses broad-spectrum, potent antimicrobial activity, 1 is highly hemolytic against mammalian erythrocytes, which precludes it from further development. In this study, we report
D A Treco et al.
Current protocols in molecular biology, Chapter 13, Unit13-Unit13 (2008-02-12)
Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.
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G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
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Thomas S Dash et al.
Structure (London, England : 1993), 27(2), 315-326 (2018-12-18)
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