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50933

Guanidine hydrochloride

Name: Guanidine hydrochloride
Brand: SIGMA

BioUltra, Molecular Biology, ≥99.5% (AT)

Synonym(s):

Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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About This Item

Linear Formula:

NH₂C(=NH)NH₂ · HCl

CAS Number:

50-01-1

Molecular Weight:

95.53

UNSPSC Code:

12352107

NACRES:

NA.28

PubChem Substance ID:

57651284

EC Number:

200-002-3

Beilstein/REAXYS Number:

3591990

MDL number:

MFCD00013026

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Properties

biological source

synthetic

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (AT)

form

powder or crystals

technique(s)

RNA extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.05% (as SO₄)

loss

≤0.05% loss on drying, 20 °C (HV)

color

colorless to white,

pH

4.5-6.0 (25 °C, 6 M in H₂O)

mp

180-185 °C (lit.)

solubility

H₂O: 6 M at 20 °C, clear, colorless

density

1.3 g/cm³ (lit.)

anion traces

sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤150 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

SMILES string

Cl[H].NC(N)=N

λ

6 M in H₂O

UV absorption

λ: 260 nm A_max: 0.03, λ: 280 nm A_max: 0.02

application(s)

advanced drug delivery

storage temp.

room temp

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

Description

General description

Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant. It is a small molecule and hydroscopic in nature.

Application

Guanidine hydrochloride (GdnHCl) has been used as a component of the phosphate buffer saline (PBS) for the incubation of Schlemm′s canal (SC) cells. It has also been used as a component of the digestion buffer for the gelation and expansion of human HeLa229 cells. GdnHCl is also used to isolate RNA.

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is involved in conformational changes and loss of enzyme activity due to inactivation of the enzyme. It is involved in the loss of activity of alkaline phosphatase (ALPase) enzyme in Haliotis diversicolor. GdnHCl functions by hampering the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It can also inactivate enzymes like papain and aminocyclase. Higher concentrations of GdnHCl leads to viral inactivation of herpes simplex virus 1 (HSV-1).

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pictograms

GHS07

signalword

Warning

hcodes

H302 + H332,H315,H319

pcodes

P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000487184

BCCN6783

0000433845

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