A25120
DEAE–Sephadex® A-25 chloride form
Synonym(s):
DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®
form
powder
technique(s)
activity assay: suitable
matrix
cross-linked dextran
matrix active group
functional group
bead size
40-125 μm (dry)
pore size
~30,000 Da exclusion limit
operating pH
2-9
capacity
3-4 meq/g ion exchange capacity
compatibility
mode of use mixed weak and strong anion exchange chromatography
Quality Level
General description
The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
Application
DEAE-Sephadex® A-25 has been used:
- to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
- in the estimation of GLS content in Chinese kale sprouts
- production and purification of endozepine from astrocytes
- to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients
Features and Benefits
- Weak anion exchanger
- Suitable for batch techniques
- High binding capacities
- Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
Legal Information
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
Shu-Kui Wang et al.
Zhonghua nan ke xue = National journal of andrology, 8(3), 173-177 (2002-12-14)
To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
William F Loomis et al.
The Journal of biological chemistry, 285(28), 21359-21365 (2010-05-11)
Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty acid metabolism and extracellularly as diazepam binding inhibitor, the precursor of endozepine peptides. Two of these peptides, ODN and TTN, bind to the GABA(A) receptor and modulate its sensitivity to
DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
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