E3024
Earle′s Balanced Salts
With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
EBSS
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About This Item
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
room temp
General description
Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).
Application
Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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