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About This Item
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
21
biological source
goat
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG (immunoglobulin G) is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Immunogen
Purified mouse IgG Fab fragment
Application
Anti-Mouse IgG (Fab specific) FITC antibody produced in goat has been used in :
- fluorescent staining
- immunocytochemistry
- immunofuorescence
Biochem/physiol Actions
Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity. The conjugate shows no reactivity with mouse IgG, Fc fragment, human, bovine, or horse serum.
IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse, or human samples.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Susann Erdmann et al.
Eukaryotic cell, 11(5), 571-589 (2012-01-03)
In this study, we undertook a functional characterization and transcriptome analysis that enabled a comprehensive study of the mating type loci of the mushroom Schizophyllum commune. Induced expression of both the bar2 receptor and the bap2(2) pheromone gene within 6
Complex regulation of spiral ganglion neuron firing patterns by neurotrophin-3
Zhou Z, et al.
The Journal of Neuroscience, 25(33), 7558-7566 (2005)
Xiuli Wang et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 24(1), 106-119 (2017-10-25)
Purpose: Multiple myeloma remains an incurable malignancy of plasma cells despite considerable advances in treatment. The purpose of the study was to develop novel chimeric antigen receptors (CAR) for the treatment of multiple myeloma and explore combinatorial therapy using CAR