G4142
β-Galactosidase from bovine testes
ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
β-D-Galactoside galactohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
1.0-3.0 units/mg protein (modified Warburg-Christian)
Biological source:
bovine
biological source
bovine
Quality Level
form
ammonium sulfate suspension
specific activity
1.0-3.0 units/mg protein (modified Warburg-Christian)
foreign activity
α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%
storage temp.
2-8°C
Related Categories
Application
β-Galactosidase from bovine testes was used for coupling to Sepharose.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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W Kiess et al.
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
J J Distler et al.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
Elena Piegari et al.
Basic research in cardiology, 108(2), 334-334 (2013-02-16)
The increasing population of cancer survivors faces considerable morbidity and mortality due to late effects of the antineoplastic therapy. Cardiotoxicity is a major limiting factor of therapy with doxorubicin (DOXO), the most effective anthracycline, and is characterized by a dilated
Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus.
Yiquan Zhang et al.
Future microbiology, 8(2), 271-280 (2013-02-05)
To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis. Primer extension, LacZ fusion, gel mobility shift and DNase I footprinting assays were conducted in combination for this gene regulation
Ronit Tokarsky-Amiel et al.
Cancer research, 73(9), 2829-2839 (2013-02-21)
Cellular senescence, a state of cell-cycle arrest accompanied by dramatic morphologic and metabolic changes, is a central means by which cells respond to physiologic stress and oncogene activity. Senescence is thought to play important roles in aging and in tumor
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