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About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
form
resin
crosslinking
cross-linked
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-5113-01
storage condition
(20% Ethanol)
parameter
4-30 °C temp. range (working)
technique(s)
liquid chromatography (LC): suitable
matrix
highly cross-linked 6% agarose
matrix active group
Streptavidin
avg. part. size
34 μm
working range
2-10.5
capacity
>300 mg binding capacity (Biotin / mL resin), 6 mg binding capacity (biotinylated BSA / mL resin)
separation technique
affinity
storage temp.
2-8°C
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Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Features and Benefits
- Streptavidin immobilized to Sepharose™ High Performance for purification of biotinylated compounds
- Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
- 34 μm bead size for improved resolution
General description
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.
Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.
Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
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We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
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