H5791
Hepatocyte Growth Factor human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
HGF, Hepatopoietin A, Scatter Factor
Product Name
Hepatocyte Growth Factor human, HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤20 ng/mL EC50
quality
endotoxin tested
mol wt
monomer 70 kDa (glycosylated)
packaging
pkg of 5x10 μg
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... HGF(3082)
Looking for similar products? Visit Product Comparison Guide
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.
Biochem/physiol Actions
HGF is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. It is a potent mitogen, stimulating the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells), and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells, and HepG2 (human hepatoma) cells. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
General description
The HGF gene spans ∼70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of human chromosome 7, 7q21.1.
Other Notes
The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
T Nakamura et al.
Nature, 342(6248), 440-443 (1989-11-23)
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification
Karène Mahtouk et al.
Biochimica et biophysica acta, 1806(2), 208-219 (2010-07-27)
Hepatocyte growth factor (HGF) and its receptor MET are essential during embryonic development and throughout postnatal life. However, aberrant MET activation, due to overexpression, mutations, or autocrine ligand production, contributes to the development and progression of a variety of human
MET and PI3K/mTOR as a potential combinatorial therapeutic target in malignant pleural mesothelioma.
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
Hiroshi Kusunoki et al.
Hypertension research : official journal of the Japanese Society of Hypertension, 37(6), 500-506 (2014-03-14)
The paracrine loop involving inflammatory cytokines between adipocytes and macrophages establishes a vicious cycle that aggravates pro-inflammatory changes in adipose tissue. The serum level of hepatocyte growth factor (HGF) is increased in metabolic syndrome, but whether HGF is beneficial or
Nina Marie Pedersen et al.
The Journal of cell biology, 219(8) (2020-06-02)
Cancer cells break tissue barriers by use of small actin-rich membrane protrusions called invadopodia. Complete invadopodia maturation depends on protrusion outgrowth and the targeted delivery of the matrix metalloproteinase MT1-MMP via endosomal transport by mechanisms that are not known. Here
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H5791-5X10UG | 04061833799826 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service