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Merck

H5912

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
MDL number:
MFCD04974783
Conjugate:
unconjugated
Clone:
polyclonal
Application:
microarray
western blot
Species reactivity:
mouse, human
Citations:
25
Technique(s):
microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
Uniprot accession no.:
P16104

Key Documents

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Product Name

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

mouse, human

technique(s)

microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine

UniProt accession no.

P16104

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer139)

Quality Level

200

Gene Information

human ... H2AFX(3014)
mouse ... H2afx(15270)

Application

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

Biochem/physiol Actions

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

Immunogen

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.
synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000409212
0000386117
0000336889
0000409212
0000386117

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Yaman Tayyar et al.
American journal of cancer research, 11(6), 3240-3251 (2021-07-13)
Human papilloma virus (HPV) is the main causative agent in cervical cancers. High-risk HPV cancers, including cervical cancer, are driven by major HPV oncogene, E6 and E7, which promote uncontrolled cell growth and genomic instability. We have previously shown that
Ruth E Gonzalez et al.
Cell cycle (Georgetown, Tex.), 10(20), 3505-3514 (2011-11-10)
Topoisomerase II (Topo II) that decatenates newly synthesized DNA is targeted by many anticancer drugs. Some of these drugs stabilize intermediate complexes of DNA with Topo II and others act as catalytic inhibitors of Topo II. Simultaneous depletion of Topo
Roderick W Kumimoto et al.
Frontiers in plant science, 12, 707923-707923 (2021-10-19)
Numerous links have been reported between immune response and DNA damage repair pathways in both plants and animals but the precise nature of the relationship between these fundamental processes is not entirely clear. Here, we report that XAP5 CIRCADIAN TIMEKEEPER
Genomic instability in mice lacking histone H2AX
Celeste A, et al.
Science (New York, N.Y.), 296(5569), 922-927 (2002)
H2AX: functional roles and potential applications
Dickey J, et al.
Chromosoma, 118(6), 683-692 (2009)
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