KMM-101NV
KOD One™ PCR Master Mix
Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR
Synonym(s):
Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One™ PCR Ready Mix
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106314
biological source
Escherichia coli (carrying the cloned UKOD DNA polymerase gene)
form
liquid
usage
sufficient for 25 reactions, sufficient for 500 reactions
feature
Fast PCR, High Fidelity PCR, dNTPs included, hotstart
technique(s)
PCR: suitable
input
crude DNA
shipped in
dry ice
storage temp.
−20°C
Application
- Genomic DNA amplification
- cDNA amplification
- Direct PCR
- Colony PCR
- Amplification of NGS libraries
- Site direct gene mutation
Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
Features and Benefits
- Fast: Amplifies the targets using the following very short conditions:
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
- Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
- High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
- Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
- High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
General description
KOD One™ PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
Legal Information
*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.
Other Notes
For R&D use only. Not for drug, household, or other uses.
Packaging
KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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