L9381
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
FITC conjugate, lyophilized powder
Synonym(s):
Bandeirea simplicifolia agglutinin, BS-I
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Bandieraea simplicifolia
biological source
Bandieraea simplicifolia
Quality Level
conjugate
FITC conjugate
form
lyophilized powder
potency
<100 μg per mL agglutination activity (using human blood group A erythrocytes)
composition
Protein, ~10% Lowry
extent of labeling
1-3 mol FITC per mol protein
storage temp.
−20°C
Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Physical form
Contains phosphate buffer salts and sodium chloride
Analysis Note
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Other Notes
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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