M1302
M-MLV Reverse Transcriptase
Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
Synonym(s):
Moloney Murine Leukemia Virus Reverse Transcriptase
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About This Item
Product Name
M-MLV Reverse Transcriptase, Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis
biological source
Porcine intestinal mucosa
recombinant
expressed in E. coli
form
liquid
usage
sufficient for 200 reactions
feature
dNTPs included: no
hotstart: no
concentration
200 units/μL
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
Application
M-MLV Reverse Transcriptase has been used:
- for the preparation of cDNA libraries or for first strand cDNA synthesis
- for use in a 2-step RT-PCR assay
- in quantitative realtime-polymerase chain reaction (RT-qPCR)
- in reverse transcription
Features and Benefits
- Thermostable reverse transcriptase active at 37 °C.
- Can be used to generate first strand cDNA of up to 7 kb.
General description
Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Other Notes
One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.
Packaging
Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.
Preparation Note
The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase.
Marie L Coté et al.
Virus research, 134(1-2), 186-202 (2008-02-26)
Recent X-ray crystal structure determinations of Moloney murine leukemia virus reverse transcriptase (MoMLV RT) have allowed for more accurate structure/function comparisons to HIV-1 RT than were formerly possible. Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
John J Docherty et al.
Antiviral research, 72(3), 171-177 (2006-08-11)
Resveratrol was found to inhibit varicella-zoster virus (VZV) replication in a dose-dependent and reversible manner. This decrease in virus production in the presence of resveratrol was not caused by direct inactivation of VZV or inhibition of virus attachment to MRC-5
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
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