MAK094
Protein Carbonyl Content Assay Kit
sufficient for 100 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
Protein Carbonyl Content Assay Kit, sufficient for 100 colorimetric tests
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases
storage temp.
2-8°C
Application
Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.
Suitable for quantifying carbonyls in protein samples
General description
Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.
Biochem/physiol Actions
The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
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