P1411
Polymyxin B-Agarose
aqueous glycerol suspension
Synonym(s):
PMB
Product Name
Polymyxin B-Agarose, aqueous glycerol suspension
biological source
synthetic (organic)
form
aqueous glycerol suspension
extent of labeling
~1 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)
suitability
suitable for chromatography
storage temp.
2-8°C
Quality Level
Application
Polymyxin B-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B-agarose has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.
Useful for removal of endotoxins from solutions
Physical form
Suspension in 50% glycerol containing 0.02% sodium azide
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Martín López et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2409-2416 (2003-02-21)
Macrophages infected with Mycobacterium tuberculosis undergo increased rates of apoptosis. Important objectives are to define the microbial factors that cause apoptosis, the mechanisms involved and the impact on infection. The 19-kDa M. tuberculosis glycolipoprotein (p19) is both cell wall-associated and
A C Issekutz
Journal of immunological methods, 61(3), 275-281 (1983-07-29)
Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process
P J Jagielo et al.
The American journal of physiology, 270(6 Pt 1), L1052-L1059 (1996-06-01)
To investigate the role of endotoxin in grain dust-induced airway inflammation, we reduced the endotoxin activity from extracts of corn dust (CDE), using three distinct methods, and determined the effect of endotoxin activity on the in vitro and in vivo
L F Weikert et al.
American journal of physiology. Lung cellular and molecular physiology, 279(2), L216-L223 (2000-08-05)
Surfactant-associated protein A (SP-A) is involved in surfactant homeostasis and host defense in the lung. We have previously demonstrated that SP-A specifically binds to and enhances the ingestion of bacillus Calmette-Guerin (BCG) organisms by macrophages. In the current study, we
Samira B R Prado et al.
Scientific reports, 10(1), 1690-1690 (2020-02-06)
Dietary fibers have been shown to exert immune effects via interaction with pattern recognition receptors (PRR) such as toll-like receptors (TLR) and nucleotide-binding oligomerization domain (NOD)-like receptors. Pectin is a dietary fiber that interacts with PRR depending on its chemical
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