RAB0377
Visfatin EIA Kit
for serum, plasma, culture supernatant and cell lysates
Synonym(s):
Visfatin ELISA Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
species reactivity
rat, human, mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, enzyme immunoassay: suitable
input
sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NAMPT(10135)
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.
Biochem/physiol Actions
Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.
General description
The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.
Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.
Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.
Immunogen
Visfatin, synthetic peptide
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
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A visfatin promoter polymorphism is associated with low?grade inflammation and type 2 diabetes.
Zhang Y-Y, et al.
Obesity (Silver Spring, Md.), 14(12), 2119-2126 (2006)
Effects of Diet and Exercise-Induced Weight Loss in Sedentary Obese Women on Inflammatory Markers, Resistin, and Visfatin.
Khanna D, et al.
Obesity and Nutrition of J, 102 (2017)
Single and Concurrent Effects of Endurance and Resistance Training on Plasma Visfatin, Insulin, Glucose and Insulin Resistance of Non-Athlete Men with Obesity.
Tayebi S M, et al.
Annals of applied sport science., 4(4), 21-31 (2016)
Matthaios G Pitoulias et al.
Cytokine, 91, 140-144 (2017-01-11)
Visfatin is an adipokine molecule acting as an essential coenzyme in multiple cellular redox reactions. The increased serum levels of Visfatin have been correlated with metabolic syndrome and endothelial homeostasis. In this study we investigate the possible relationship of Visfatin
Ashleigh M Philp et al.
International journal of molecular sciences, 22(13) (2021-07-03)
Obesity increases the risk of hip osteoarthritis (OA). Recent studies have shown that adipokine extracellular nicotinamide phosphoribosyltransferase (eNAMPT or visfatin) induces the production of IL-6 and matrix metalloproteases (MMPs) in chondrocytes, suggesting it may promote articular cartilage degradation. However, neither
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